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Current Meetings |
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October 2011 Meeting |
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"Innovative Technologies for HPLC, GC, and Sample Prep" |
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CSSC is pleased to announce the October 2011 CSSC meeting will be held together with Supleco seminar at the Four Points by Sheraton Meriden, Meriden CT. The meeting will feature five technical presentations sponsored by Supleco and CSSC, as well as lunch and dinner. |
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Registration deadline is Wednesday, October 19, 2011. To use the online system, you must be
registered as a user. |
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Date: |
Wednesday, October 19, 2011 |
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Location: |
Four Points
by Sheraton Meriden |
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Speakers: |
Dr. Hillel Brandes, Supelco |
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Agenda: |
12:00 (noon) Registration and Lunch 1:15
- 2:40 pm “HPLC,
LC-MS and Sample Prep for LC: Techniques to Improve
Speed, Resolution, and
Sensitivity without Investing
in Capital Equipment” Presented by Hillel Brandes,Ph.D.,
Principle Applications Chemist, Supelco,
Bellefonte, PA
LC &
SFC Enantiomeric Separations” and “Ionic Liquids
in GC (Including Rapid Water Analyses) and the
Ultra-Sensitive ESI-MS of Anions” Presented
by Zachary
S. Breitbach, Ph.D., University of Texas at Arlington, Arlington, TX 3:45
- 4:30 pm “Mechanism
of Enantioseparation on Macrocyclic Glycopeptide
Chiral HPLC & CE Phases”
Presented by Shengli
Ma, Ph.D., Boehringer Ingelheim
Pharmaceuticals,
Inc., Ridgefield, CT 4:35
- 5:00 pm “Solid Phase Microextraction (SPME): Technology Overview
and Recent Advances using SPME in Bioanalysis”
Presented by Hillel Brandes 5:15 – 6:00 pm “Transfer of Biomarker Assays from RIA and ELISA to LC/MS/MS”
Presented by Kimberly
Navetta, Pfizer, Groton
Laboratories, Discovery Safety Evaluation, Groton,
CT 6:00 PM. Complimentary dinner provided by
the Connecticut Separation Science Council (CSSC). |
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Cost: |
Free—You must Register at one of the
sites below |
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Registration: |
To
Register, for the Half day session, Visit the Sigma-Aldrich Website www.sigma-aldrich.com/analytical-seminars To register
ONLY for the dinner meeting (with Technical presentation beginning at 5:15
pm) visit the CSSC Site: http://www.cssconline.org/Meetings/Current_Meetings.asp |
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LC, LC-MS and Sample Prep for
LC: Techniques to Improve Speed, Resolution, and Sensitivity without
Investing in Capital Equipment This
talk describes recent innovations in particles, sorbents, surface chemistries,
column/device formats, and reagents for HPLC and sample prep that address the
need for higher throughput, better sensitivity, and greater resolution.
Intended to be instructional, the talk will explain the scientific principles
behind today’s innovative technologies, such as Fused-Core particles,
HybridSPE-Phospholipid technology for the low-level MS detection of small
molecules in serum, solid phase microextraction (SPME), which has now been
applied to bioanalysis, molecularly imprinted polymer SPE (SupelMIP), and
others. Applications will be presented that demonstrate the utility of these
innovations in pharma, food, forensics, environmental, bioanalysis, and other
areas using interesting case studies based on the registrants’ areas of interest. Part 1: Advances in Macrocyclic
Chiral Stationary Phases for LC & SFC Enantiomeric Separations Chiral
macrocyclic molecules are one of the most important classes of chiral
selectors known. They dominate GC and CE enantiomeric separations, as well as
reversed phase and polar organic mode LC separations.
This class of selectors will be briefly reviewed and the latest exciting
results presented. The newest and most unique member of this family,
cyclofructans, will be introduced. If time allows, new chiral stationary phases based on synthetic chiral
polymers will be discussed as well. Part 2: Ionic Liquids in GC
(Including Rapid Water Analyses) and the Ultra-Sensitive ESI-MS of Anions The
first new class of GC stationary phases in 30 years are
based on ionic liquids (ILs). They have unique selectivities,
higher thermal and chemical stabilities than any of the known commercially
available columns. We will describe
them and their use in: the rapid, facile water analysis of virtually any
solvent and/or beverage, high temperature
separations, FAME isomer separations, separations with an air carrier gas, GC x GC, and other applications. ILs with
multifunctional cations also can be used as a trace additive for the ultra
sensitive detection of anions in the positive mode of ESI-MS. The mechanism and use of this novel technique will be described for both
organic and inorganic anions. Mechanism of Enantioseparation
on Macrocyclic Glycopeptide Chiral HPLC and CE Phases Macrocyclic
glycopeptides are a class of compounds often used for separation of
enantiomers via either HPLC or CZE. Two such macrocyclic glycopeptides are
teicoplanin and teicoplanin aglycone, and our
work focused on their chromatographic behavior. These compounds are
immobilized on silica matrixes and are commercially available under the trade
names Astec CHIROBIOTIC T2 and TAG, respectively. The separation of propranolol was performed on both chiral
stationary phases. A reversal of elution order was obtained when the compound
was separated on CHIROBIOTIC T2, compared with
TAG. In order to understand this behavior, vibrational circular dichroism was
undertaken in different solvents and at different temperature. The
presentation will describe all the changes occurring with these two macrocycles under the experimental conditions. Solid Phase Microextraction
(SPME): Technology Overview and Recent Advances using SPME in Bioanalysis This
talk will describe a new Solid Phase Microextraction (SPME) fiber probe that
permits the extraction of small molecule analytes directly from biological
fluids and other complex matrixes. It comprises a
metal-based fiber core coated with mixed-mode HPLC particles (C18/SCX)
embedded in a proprietary biocompatible binder. Large macromolecules, such as proteins and glycolipids, are
repelled, while smaller analytes,
such as drugs and metabolites, bind to the particles and are extracted.
Because SPME is concentration dependent rather than volume dependent, sample
volumes down to 100 μL can be extracted
and desorbed with as little as 50 μL. This is an advantage with precious
samples, like plasma from neonates, transgenic mice, and other small mammals.
The device is compatible with common laboratory robotics equipment, and allows
simultaneous extraction or desorption of multiple samples for high throughput
sample prep. Transfer of Biomarker Assays
from RIA and ELISA to LC/MS/MS The
Biomarkers’ Laboratories at Pfizer have initiated the replacement of some
ELISA and RIA kits for small molecule biomarkers with mass spectrometry (MS)
assays. When a direct comparison was conducted, the
MS assays demonstrated three major benefits over the kits: lower costs,
shorter delivery times, and higher levels of specificity. For example, the MS assay for aldosterone costs ten times
less and data reports are
generated 2 days faster. Corticosterone was moved to
the MS assay not only for the cost savings, but the reduction in sample
volume requirements. When evaluating the specificity of the kits for small molecules, many antibodies used
cross-reacted with molecules of similar structures, potentially providing
false readings. To date, three MS assays have been deployed to portfolio programs in place of their respective kits
(corticosterone, aldosterone and 25-OH Vitamin D3), and other assays are
being evaluated for future deployment. As MS is becoming established in the
clinical setting, this platform is
broadening its impact in clinical pathology applications, enhancing drug
development. These changes not only provide substantial cost and time
savings, but they provide
higher quality
data for project teams.. Complete
details of the event can also be found at www.sigma-aldrich.com/analytical-seminars |
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Directions: Click Here for Directions Four
Points by Sheraton Meriden Phone: (203) 238-2380 |
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Last Updated: 09/23/2011 01:10 PM |
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