September 2009 Meeting

"Impact of ultra performance liquid chromatography (UPLC) on proteomic research".

The September 2009 CSSC meeting will be held at the Four Points by Sheraton Meriden, Meriden CT.  The meeting will feature a technical presentation by Dr. Christopher M. Colangelo, Director of Protein Profiling, W.M. Keck Foundation Biotechnology Resource Laboratory, Yale University, as well as dinner. The cost of the meeting is $30 ($15 Students and Emeritus) and is to be paid at the event.

This meeting will be the CSSC annual meeting and a vote for the Officers of Board of Directors and the Board of Directors will be held at the meeting

Registration deadline is Tuesday, september 30th 2009 .

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Abstract:  

"Impact of ultra performance liquid chromatography (UPLC) on proteomic research"

Christopher M. Colangelo and Kathryn L. Stone

W.M. Keck Biotechnology Resource Laboratory, Yale University, New Haven, CT 06511

Mass Spectrometry, specifically LC-MS/MS, has become the de-facto detection technology for Proteomics and Protein Profiling Analyses.   In proteomics, particularly biofluid analysis (e.g. plasma), dynamic range and sensitivity of detection are critical.  Hence, proteomics has become one of the driving forces in the evolution of new HPLC technologies.  In fact, one of the first applications of a more sensitive chromatography system, nanoflow, was the coupling of a nanoflow device to an electrospray mass spectrometer for the separation and identification of Cytochrome C peptides after tryptic digestion (1).  Given this successful union of HPLC, mass spectrometry and proteomics, it comes as no surprise that the recent introduction of commercial ultra high pressure or ultra performance liquid chromatography (UPLC) systems have made great impacts on improving proteomic analyses. These LC systems have an improved resolution, speed and sensitivity and operate at ultra high pressures of up to 10,000 psi with advanced stationary phase packing materials for columns (moving from 10 µm to 3 µm and now 1.7 µm particle sizes)(2).   Data will be presented on the integration of these nanoflow UPLC systems across the Keck MS and Proteomic Biotechnology laboratory and its impact on our proteomic workflows.

Acknowledgments
            Funded with Federal funds from NHLBI/NIH contract N01-HV-28186, NIDA/NIH grant 1 P30 DA018343-01, and NIAD/NIH grant 5 U54 AI057158-02 (Northeast Biodefense Center - Regional Centers of Excellence).

(1) Davis, M.T., Stahl, D.C., Hefta, S.A., Lee, T.D. (1995) A Microscale Electrospray interface for On-Line Capillary Liquid Chromatography/ Tandem Mass Spectrometry of Complex Peptide Mixtures. Anal. Chem. 67, 4549-4556.

(2) Churchwell, M.I., Twaddle, N.C., Meeker, L.R. and Doerge, D. R. (2005) Improving LC-MS sensitivity through increases in chromatographic performance:  Comparisons of UPLC-ES/MS/MS to HPLC-ES/MS/MS.  J. Chromatog. 825, 134–143.

Biography:

Christopher Colangelo, Ph.D., Director of Protein Profiling, W.M. Keck Foundation Biotechnology Resource Laboratory, Yale University.
For the past 7 years, Dr. Colangelo has overseen the protein profiling core of the Keck MS Resource.  During this time he worked with hundreds of investigators to educate, design, and analyze their protein profiling samples. In addition he has overseen the implementation of four new protein profiling technologies into the Keck Foundation Biotechnology Resource Laboratory, namely iTRAQ, ICAT, MudPIT, and Targeted Proteomics. . Dr. Colangelo has ten years of mass spectrometry experience, using MALDI-TOF (Applied Biosystems DE-Pro, Bruker Reflex, and Micromass M@LDI-LR, Applied Biossytems 4700 TOF-TOF) and nanospray ESI-MS/MS (Thermo Finnigan DecaXP, Thermo Finnigan LCQ Classic, Micromass Q-TOF, Applied Biosystems QSTAR XL, Appiled Biosystems QSTAR Elite, Applied Biosystems 4000 QTRAP, and Applied Biosystems API 5000). During his graduate career he helped develop a novel technique for whole cell MALDI analysis which enables the monitoring of protein expression in whole cell bacteria (Easterling, et. al., Anal Chem. 1998, 70, 2704-09). In 2000, Dr. Colangelo was hired by CuraGen Corporation to work in the Advanced Engineering group where he developed new technologies for both genomic and proteomic analysis, work for which he obtained patent applications in both areas. Recently he was an author of two book chapters on proteomics, 17 peer-reviewed publications, and has been an invited speaker at 7 international meetings.

Four Points by Sheraton Meriden
275 Research Parkway
Meriden, CT 06450

Last Updated:  08/16/2009 03:13 PM