October 2008 Meeting

New Fused-core® Column Packings for Fast HPLC Separations.

The October 2008 CSSC meeting will be held at the 95 Gathering Place in Wallingford, CT.  The meeting will feature a technical presentation by Dr. Joseph J. DeStefano of Advanced Materials Technology, Inc., Wilmington, DE as well as dinner. The cost of the meeting is $30 ($15 Students and Emeritus) and is to be paid at the event. This meeting will be the CSSC annual meeting and a vote for the Officers of Board of Directors and the Board of Directors will be held at the meeting.

Registration deadline is Thursday, October 30th 2008 .

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Abstract:  

Joseph J. Destefano, PhD

Advanced Materials Technology, Inc., Wilmington, DE 19810

Columns of superficially porous particles with an overall diameter of 2.7 μm and a porous shell of 0.5 μm thickness have been shown by many users to possess unusual efficiency and stability, allowing very fast separation speeds with ruggedness of operation. These fused-core particles exhibit resolution and separation speeds competitive with sub-2 μm totally porous particles but with one-half to one-third the column back pressure. The current popularity of fused-core particle columns is based on the widely-used C18 and C8 bonded phases. Separations by reversed-phase HPLC are most often performed using C18 bonded phase columns.  The reasons for this popularity are 1) the C18 phase was one of the first reversed-phase packings in the commercial market, 2) the C18 phase has a reputation for being a very robust phase, and 3) there is a vast library of applications on the C18 phase on which to base starting conditions for new applications.  Other reversed phase HPLC columns such as cyanopropyl, phenyl, amino, etc. are used significantly less often in published applications, but they do provide chromatographers with alternate selectivities for compounds relative to C18 phases.  New separation capabilities for fused-core columns now are available with other stationary phases to widen the separation selectivity possibilities. The addition of an unbonded silica, used for normal-phase or hydrophilic interaction (HILIC) separations, and a new, stable polar-embedded phase allow alternate separation selectivities that enhance the use of the high-efficiency fused-core particles for very fast, rugged separations. Illustrative separations with these new fused-core materials and the effects of operating variables such as mobile phases, flow rate, and temperature demonstrate the effectiveness of these new stationary phases for selectivity enhancement.

Biography:

Dr. DeStefano received his B.A. degree in chemistry from the University of Connecticut in 1965.  He then joined the DuPont Company later that year and began his studies at the University of Delaware leading to M.S. and Ph.D. degrees in Analytical Chemistry in 1968 and 1972, respectively.  He spent 25 years working in various scientific and managerial positions in the DuPont Company.  He left DuPont in 1990 to co-found and serve as President of Rockland Technologies, Inc., a company licensed by DuPont to produce and sell the Zorbax brand of HPLC columns and packings.  In 1997, Rockland was acquired by the Hewlett-Packard Company, and Dr. DeStefano took on the title of Zorbax Business Manager and provided technical expertise for the manufacture of HPLC columns and packings for HP.  Hewlett-Packard divided into two companies in 1999, and Dr. DeStefano remained with that part of the original Hewlett-Packard Company that manufactures and markets their analytical equipment, Agilent Technologies, Inc., until 2005.  Currently, Dr. DeStefano is President of Advanced Materials Technology, Inc., a HPLC columns and packings manufacturing company he founded with co-investors in 2005.  AMT is the developer and manufacturer of the novel superficially porous packing material trade named HALO.

Dr. DeStefano was an early contributor to the development of HPLC columns and packings that later resulted in HPLC becoming a major tool for the separation of mixtures of compounds by liquid-phase methodology.  His collaboration with Dr. Jack Kirkland, one of the pioneers of the HPLC technique, led to the utilization of totally porous silica microparticles and mono-functional silanes in modern HPLC.  He was instrumental in implementing the first computerized manufacturing quality assurance system that became a model for every HPLC column sold to have its own test report included with the column.  Dr. DeStefano was also the supervisor of the HPLC and Thermal Analysis instrument engineering groups, developing several new generations of these instruments for the Instrument Products Group at DuPont.  Later in his career, Dr. DeStefano was the Business Manger for three product lines at DuPont:  HPLC columns and consumables, Sorvall-brand centrifuges, and the Service business for the centrifuge product line.

Dr. DeStefano has authored or co-authored more than thirty major research publications in gas and liquid chromatography.  He is also a member of the Scientific Committee of the International Symposium for the Separation of Proteins, Peptides, and Polynucleotides and has been chairman and organizer for the scientific conferences held in the U.S. by the ISPPP since 1997.

95 Gathering Place
865 North Colony Road
Wallingford, CT 06492
(203) 265-1552

Last Updated:  10/11/2008 08:34 PM