October 2007 Meeting

Protein Separations in Chromatographic Systems:
A-priori Prediction of Protein Affinity,
Selective Displacers and Generic Antibody Purification Processes

The October 2007 CSSC meeting will be held at the George's II Restaurant in Wallingford, CT.  This meeting will be the CSSC annual meeting and a vote on the 2007 Officers and members of the Board of Directors will be held at the meeting. The meeting will also feature a technical presentation by Dr.Steven Cramer of Rensselaer Polytechnic Institute, as well as dinner.  The cost of the meeting is $30 ($15 Students and Emeritus) and is to be paid at the event.

The registration deadline is October 16.

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Abstract:  

A multi-scale modeling approach is presented for the a priori prediction of protein retention, isotherm parameters and preparative chromatographic behavior in ion-exchange and hydrophobic interaction systems. Predictive QSPR models were generated for isotherm parameters using novel pH sensitive molecular descriptors and SVM learning algorithms.
The important molecular property descriptors were examined to provide insight into selectivity in these systems. The predicted isotherm parameters were employed in column chromatography models to successfully predict column performance under a range of operating conditions (salt, pH, mode, resins). SVM classification models were also used in concert with high throughput experimentation to develop highly selective displacers for the separation of difficult protein mixtures.
These new classes of displacers enable the separation of similar proteins using low efficiency or even batch chromatographic systems. Finally, fundamental studies were carried out on antibody purification using various protein A ligands and resin materials to provide insight into selectivity and dynamic capacity differences in these materials. These developments represent the state-of-the-art in structure-property modeling as applied to chromatography and can shed light into the nature of selectivity in various chromatographic systems.

Biography:
  Dr. Steve Cramer is the William Weightman Walker Professor of Chemical and Biological Engineering at Rensselaer Polytechnic Institute, Troy, New York.

Dr. Cramer is conducting research on several areas related to chromatographic bioprocessing including: rational design of high affinity and chemically selective displacers for protein and oligonucleotide purification in ion exchange and hydrophobic displacement systems, multi-scale modeling of complex chromatographic behavior, molecular modeling of selectivity and affinity in chromatographic systems, spectroscopy/chromatographic investigation of protein-surface interactions, chromatography on a chip and novel separation systems for proteomics.
He has won several awards including: the 2006 Alan S. Michaels Award for the Recovery of Biological Products (ACS Division of Biochemical Technology); School of Engineering Research Excellence Award, RPI; Presidential Young Investigator, National Science Foundation; and the Early Career Award, RPI. He has delivered major keynote and plenary addresses at a number of International Conferences, is a Fellow of the American Institute for Medical and Biological Engineering and is the Editor of the journal Separation Science and Technology.

George's II
1033 North Colony Road
Wallingford, CT 06492
(203) 269-1059

Last Updated:  09/10/2007 08:33 PM