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October 2002 Meeting |
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Strategies for the Identification of Metabolites Using Hybrid Quadrupole-Linear Ion Trap Mass Spectrometer |
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The October 2002 Connecticut Separation Science Council was a joint meeting with the Connecticut Mass Spectrometry Discussion Group (CTMSDG). |
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Date: | Tuesday, October 15, 2002 | |||||||
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Location: | Villa
Capri Banquet Facility Wallingford, CT |
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Speaker: | Yeves Le Blanc, Ph. D. | |||||||
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Agenda: | 5:30-6:30
PM Registration/Social Hour 6:30-7:30 PM Dinner 7:30-8:30 PM Presentation |
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Cost: | $25 ($15 Students and Emeritus) | |||||||
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Registration Deadline: |
Friday, October 11, 2002 | |||||||
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| Abstract: The rapid identification of metabolites from in-vivo and in-vitro sample plays a major role in the drug discovery process, but remains challenging when non-radiolabelled and low sample volumes are provided. Strategies involving multiple MS instrument platforms have been proposed to address these challenges. This approach combines the strength of each MS instrument type to provide a complete picture of the metabolites. Identification utilizing the specificity of triple quadrupole instrument (precursor and neutral loss scans) followed by MSMS confirmation using more sensitive systems such as ion trap, is a commonly used strategy. However, this approach implies that multiple LC/MS set-ups are required which may lead to problems associated LC reproducibility in addition to increases in analysis time. With the advent of hybrid quadrupole linear ion trap (Q TRAP), identification and confirmation of the metabolites can be obtained in a single injection on a single instrument. With Information Dependent Acquisition (IDA) the metabolite can be automatically identified using the system quadrupole scans and confirmed with the high sensitivity MSMS scan of the linear ion trap. In cases where ambiguity still prevails, accurate mass measurements obtained from hybrid quadrupole time-of-flight instrument (QStar) can provide the desired confidence in the metabolites. Combination of Q TRAP and QStar information yield a complete picture of the metabolites that may exist while minimizing the number of required set-up and data manipulation and training since common software are used. | |||||||||
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| Biography: Dr. Le Blanc obtained his Ph.D. from Queens University in Kingston in collaboration with the National Research Council (Ottawa) studying the fundamentals of electrospray ionization mechanism. In 1995, he joined MDS Sciex as an application chemist for the API 300/365 product line, supporting mainly quantitative applications in LC/MSMS. He was involved in the research work that lead to the development of the API 3000. Dr. Le Blanc joined the Phoenix International (now MDS Pharma Services) in 1998 as the manager for the LC/MS instrument laboratory in the bioanalytical group. In 1999, he re-joined the Applied Biosystems/MDS Sciex Company as part of the product application laboratory in Toronto, first as a manager and later as a technical marketing scientist. In the last 2 years, Dr. Le Blanc has been involved in the development of the QTRAP instrument by providing support to applications for metabolism and proteomic, as well as defining software and instrument specifications in each of these fields. | |||||||||
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| Directions:
Traveling North or South I-95 (from
Rhode Island or New York): Traveling
South I-91: Traveling
North or South on the Merritt or Wilbur Cross Parkway (Rt. 15): Traveling
from Rt. 86 from Boston: Traveling
from Rt. 84 from Danbury: |
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