December 2004 Meeting

CSSC Annual Meeting

Kinetic Analysis and Subambient Temperature Chromatography

The December 2004 CSSC meeting will be held at the Southbury Hilton in Southbury, CT,
and will include our annual election of officers.
Registration will begin on November 1, 2004.
To register, use our secure online system by clicking here.  
To use the online system, you must be registered as a user.
Clicking here to become a registered user. 

Advances in liquid chromatography, including the synthesis of stationary phases with controlled pores and particle sizes, along with the development of chemically bonded phases, have led to explosive development in the field of analytical chemistry in the last decade. The speed, high efficiency, and easily manipulated selectivity of modern chromatographic systems continue to make this technique directly applicable in various fields. Production, identification, and impurity profile characterization of new compounds is performed routinely, consistently, and efficiently through the use of these chemically bonded phases.

Chromatography has been applied in the pharmaceutical industry as a powerful analytical method for the determination of impurity profiles. However, when establishing the purity of a drug, care must be taken in interpreting chromatographic zones, since not all of them may be due to impurities. Some chromatographic zones may be artifacts of the system related to degradation of the sample during the chromatographic process. 

There are various sources of multiple zones in chromatography: chemical reactions, impurities present in the sample solution, discontinuities in the stationary and mobile phases, formation of charged species and/or complexes and equilibrium between species.

It has been long recognized that a chemical reaction occurring during a chromatographic process can give rise to distorted peaks and breakthrough curves. Clearly, determining the kinetics of such reactions can help to establish proper chromatographic conditions so that extraneous peaks will be minimized. An undesired reaction may be mitigated by lowering the temperature at which separation occurs.

Examples of on-column reaction and the possibility of controlling the rates of such reactions will be presented in this talk. The determination of the kinetic parameters and their usefulness in optimizing the chromatographic separation will underline their importance to controlling the separation.

I-84 East: Take Exit 16. At the bottom of the exit ramp, take a right. After you pass through 2 traffic lights, the Southbury Hilton will be on your right.

I-84 West: Take Exit 16. At the bottom of the exit ramp, take a right. After you pass through 1 traffic light, the Southbury Hilton will be on your right.

Southbury Hilton
1284 Strongtown Road
Southbury, Connecticut 06488