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December 2002 Meeting |
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Recent Developments in Preparative ChromatographyA.
Chemical and Operational Considerations for Increased Load and Speed
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Date: | Wednesday, December 4, 2002 | |||||||
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Location: | Pfizer
Global Research and Development Groton Laboratories -- Groton, CT |
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Speaker: | Thomas
E. Wheat, Ph.D. Waters Corporation |
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Agenda: | 5:30-6:30
PM Registration/Social Hour 6:30-7:30 PM Dinner 7:30-8:30 PM Presentation |
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Cost: | $25 ($15 Students and Emeritus) | |||||||
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Registration Deadline: |
Monday, December 2, 2002 | |||||||
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| Abstract:
Preparative chromatography is most properly
defined as a technique for isolating some compound for use in another
experiment. It is critical in many types of research and development
ranging from characterizing small amounts of metabolites or breakdown
products up to purifying large enough quantities for biological or other
use testing. In all cases, the modern emphasis is shifting to performing
the separations more and more quickly and processing the fractions more
efficiently. Over the last few years, we have been learning, and often
re-learning, the ways in which basic principles of separation chemistry
can be applied to problems of isolation and purification. The experiments
described focus on the factors that limit loading capacity, and therefore,
speed and resolution. The effect of sample ionization and how it may be
managed provides several useful approaches. In addition, some unexpected
effects limiting capacity will be considered, as well as techniques for
obviating them.
In the course of studying techniques for increasing loadability, it became apparent that the issue of detector sensitivity in prep needed reconsideration if the separation chemistry were to be fully exploited. The several considerations in arranging useful detection using both UV detectors and mass spectrometers will be discussed. |
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| Biography: Thomas E. Wheat received a B.A. biology from the College of St. Thomas and a Ph.D. in Cell Biology from the University of Illinois, working in biochemical genetics of fish populations. He was a post-doctoral fellow and research associate at Northwestern University, in reproductive immunology. The work involved characterizing protein structure as related immunological properties. He joined Waters as an application chemist working on the analysis and purification of proteins, peptides, amino acids, and oligonucleotides. He then was employed at ThermoBioAnalysis, where he specialized in capillary electrophoresis, especially in conjunction with mass spectrometry. He rejoined Waters as part of the North American/South American LC/MS group. His work focuses on several specialty applications including mass- and UV-directed autopurification. | |||||||||
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| Directions:
From I-95 North: Take Exit 87 (Clearance B. Sharpe Highway Rt. 349). Continue through two traffic lights (stay in Left lane). The road will narrow to one lane each way. At the second stop sign, Pfizer will be on the right. Do not proceed to the guard station. Take a left into visitor’s parking. Check into the Visitor’s Center. You must be on the guest list to gain entry. A bus will take you to the Building 200 Cafeteria. From I-95 South: Take Exit 87 (Clearance B. Sharpe Highway Rt. 349). Continue through two traffic lights (stay in Left lane). The road will narrow to one lane each way. At the second stop sign, Pfizer will be on the right. Do not proceed to the guard station. Take a left into visitor’s parking. Check into the Visitor’s Center. You must be on the guest list to gain entry. A bus will take you to the Building 200 Cafeteria. Pfizer Global Research and Development Visitor’s Center Phone (860) 441-4000 |
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