April 2002 Meeting

“Mini-Scout” and “LC-MS” Kits:  Practical Examples of the Use of “Multi-Phase HPLC Method Development Kits” to Aid Rapid Generic Method Development for Both QC and R&D Applications

The concept of using "Mini-Scout Kits" (75 x 4.6 mm id columns), "LC-MS Kits" (50 x 3.0 mm id columns), "Mini LC-MS Kits" (20 x 4.0 mm id columns) and "Micro LC-MS Kits" (20 x 2.0 mm id columns ) of different selectivities and then linking these novel affinities in series using the "Super-Linker" to achieve unique "Multi-Mode" separations, is detailed.

A "Kit" contains 5 columns, each having a different selectivity. An appropriate selection of 5 phases from over 20 different phases can be chosen. These phases may be based on any of 3 different silicas or di-vinyl benzene, spherical five-micron particles. The short "Kit" columns, when combined with a generic linear-gradient procedure, produce very rapidly separations (5 to 10 times faster than standard analytical columns) and define retention maps for various selectivities allowing logical prediction of appropriate conditions.

Phases will be described that vary carbon chain length, carbon load, functionality, extent of end capping, pore size, etc., on a single base 5 micron particle. This varied selectivity of phase gives "Kit" users a unique insight into the fundamental chromatographic processes controlling their separation. The value of this technique is shown using chromatographically relevant probe mixes of difficult to resolve neutral, acid and basic compounds. These probes are specifically chosen to cover different structures and a large octanol-water partition coefficient range. The results graphically display both the merits of having different reverse phase materials in a chromatographer's armory and the value of mixed "Multi-Mode" selectivities.

The choice of isocratic/gradient conditions: single phase; "Multi- Mode" phases and the ultimate selection of an analytical/prep column's length/diameter can be predicted from the rapidly achieved "Kit" results. This concept is detailed and rationalized so as to act as a "Redi-Reckoner" that allows chromatographers to easily predict appropriate analytical, lab-prep or process-prep chromatographic conditions for their compounds.

I-84 West  Take Exit 16.  At the bottom of the exit ramp, take a right.  After you pass through 2 traffic lights, the Southbury Hilton will be on your right.

I-84 East  Take Exit 16.  At the bottom of the exit ramp, take a right.  After you pass through 1 traffic light, the Southbury Hilton will be on your right.

Southbury Hilton
1284 Strongtown Road
Southbury, Connecticut 06488